mouse collagen ii ab 3 antibody Search Results


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R&D Systems antibody against ssea 1
Antibody Against Ssea 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Oncogene Science Inc anti-p53 antibody
Anti P53 Antibody, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore mouse anti-porin 31-hl (ab-3)
Mouse Anti Porin 31 Hl (Ab 3), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse anti-pr ab-3 (catalog number ms-193-p0)
Mouse Anti Pr Ab 3 (Catalog Number Ms 193 P0), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse anti-egfr (ab-3
Mouse Anti Egfr (Ab 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore mouse mab (ab-3
Mouse Mab (Ab 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Upstate Biotechnology Inc antibody against nitrotyrosine
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Antibody Against Nitrotyrosine, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore mouse anti-pan-ras (ab-3)
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Mouse Anti Pan Ras (Ab 3), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology mouse monoclonal antibodies cm2b4
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Mouse Monoclonal Antibodies Cm2b4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse monoclonal anti-cyclin b1 (ab-3, clone gns1)
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Mouse Monoclonal Anti Cyclin B1 (Ab 3, Clone Gns1), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex mouse monoclonal fcrn-ab gtx14550 antibody
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Mouse Monoclonal Fcrn Ab Gtx14550 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore anti-pan-ras (ab-3) mouse monoclonal antibodies
Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with <t>nitrotyrosine</t> (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.
Anti Pan Ras (Ab 3) Mouse Monoclonal Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with nitrotyrosine (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Nitration of specific tyrosines in F o F 1 ATP synthase and activity loss in aging

doi: 10.1152/ajpendo.00739.2009

Figure Lengend Snippet: Immunoprecipitation of nitrated proteins from mitochondria. Mitochondrial fractions [total (T), inner membrane (IM), contact sites (CS)] were immunoprecipitated with nitrotyrosine (A and B) or β-subunit antibodies (C and D). Western blots of the immunoprecipitated fractions were then probed for either nitrotyrosine (A and C) or β-subunit (B and D) antibodies. Blots in A and C were blocked with nonfat dry milk (NFDM) with the addition of F(Ab)2, whereas blots in B and D were blocked with NFDM only. This control was done to identify the band appearing at 63 kDa as the heavy chain of immunoglobulins from the immunoprecipitation procedure. E: l-argininne-supplemented rat liver mitochondria isolated from young rats were incubated for 0, 5, 15, 30, and 60 min (●). Samples at each time point were separated by two-dimensional gels, probed for nitrotyrosine, and then stripped and probed for the β-subunit by using Western blots. Control experiments were performed by incubating mitochondria with NG-monomethyl-l-arginine (○), an inhibitor of nitric oxide (NO) synthase. MWM, molecular weight markers.

Article Snippet: Mitochondrial fractions [T (total mitochondria), IM, and CS], each containing 100 μg of protein, were incubated for 1 h with either 2.5 μg/ml of antibody against ATPase β-subunit (catalog no. 612518; BD Biosciences) or 50 μg/ml of antibody against nitrotyrosine (mouse antibody, catalog no. 05-233; Upstate Biotechnology).

Techniques: Immunoprecipitation, Western Blot, Isolation, Incubation, Molecular Weight

Effect of β-subunit nitration on ATP synthase activities

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Nitration of specific tyrosines in F o F 1 ATP synthase and activity loss in aging

doi: 10.1152/ajpendo.00739.2009

Figure Lengend Snippet: Effect of β-subunit nitration on ATP synthase activities

Article Snippet: Mitochondrial fractions [T (total mitochondria), IM, and CS], each containing 100 μg of protein, were incubated for 1 h with either 2.5 μg/ml of antibody against ATPase β-subunit (catalog no. 612518; BD Biosciences) or 50 μg/ml of antibody against nitrotyrosine (mouse antibody, catalog no. 05-233; Upstate Biotechnology).

Techniques: Nitration